Data · dataset · 2026
Raw Western blot data of baicalein inhibiting MAPK/ERK-FOS pathway in intervertebral disc degeneration
Listed in ScienceDB
This dataset contains raw Western blot (WB) images supporting the research article "Baicalein ameliorates intervertebral disc degeneration by inhibiting the MAPK/ERK-FOS pathway".
Description
Data are organized by figure (Figure 3-5, 7) and include 13 target proteins: GAPDH (loading control), COL2A1, Bcl-2, Caspase-3, Bax, FOS, ERK1/2, p-ERK1/2, MEK1/2, p-MEK1/2, c-Raf, p-c-Raf (Ser338), and Ras.Experimental design: Figure 3: human degenerated vs. normal NP tissue (n=3).
Figure 4: TNF-α-treated human NP cells (n=3). Figure 5: baicalein treatment and FOS overexpression in TNF-α-stimulated NP cells (Control, TNF-α, TNF-α+baicalein 50 µM, TNF-α+baicalein+oeFOS; n=3). Figure 7: rat caudal disc degeneration model (Sham, AFP, AFP+GelMA, AFP+Bai@GelMA; n=3).
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Methods
Protein extracts were separated by SDS-PAGE, transferred to PVDF membranes, and probed with primary antibodies from Abcam (GAPDH ab8245, COL2A1 ab307674, Bcl-2 ab182858, Caspase-3 ab184787, Bax ab32503, FOS ab208942, ERK1/2 ab184699, p-ERK1/2 ab201015, MEK1/2 ab178876, p-MEK1/2 ab194754, c-Raf ab137435, Ras ab52939) and Cell Signaling Technology (p-c-Raf Ser338 #9427). Molecular weights were estimated using PageRuler Prestained Protein Ladder (Thermo, 26617).
Signals were detected by BeyoECL Plus (P0018S) on ChemiDoc Go (Bio-Rad).File organization: Files are named as Fig{X}_{group}_{protein}_{replicate}.Tif. README.md provides detailed molecular weight annotations and explanations for all non-specific bands.Data processing: Band intensities were quantified using ImageJ. Phosphorylated protein levels were normalized to total protein and GAPDH.
No data are missing. All raw images are unmodified full membrane exposures.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.010xx ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Image 65%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.010xx | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |