Data · dataset · 2015
RNAi-mediated gene silencing of WsSGTL1 in W.somnifera affects growth and glycosylation pattern
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Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol–glucosides (SGs) in many organisms.
Description
WsSGTL1 is a SGT of Withania somnifera that has been found associated with plasma membranes. However its biological function in W.somnifera is largely unknown.
In the present study, we have demonstrated through RNAi silencing of WsSGTL1 gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic W.somnifera . For this, RNAi construct (pFGC1008- WsSGTL1 ) was made and genetic transformation was done by Agrobacterium tumefaciens . HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of W.somnifera ) and a large increase of withanolides (majorly withaferin A) content.
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Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of WsSGTL1 gene in W.somnifera .
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- Repository landing page tandf.figshare.com/articles/dataset/RNAi_mediated_gene_silencing_of_i_WsSGTL1_i_i… ↗
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- DOI doi.org/10.6084/m9.figshare.1569161 ↗
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Documentation and papers
- Creative Commons Attribution 4.0 International creativecommons.org/licenses/by/4.0/legalcode ↗
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- IsSupplementTo 10.1080/15592324.2015.1078064 doi.org/10.1080/15592324.2015.1078064 ↗
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Topics
- Stated by source
- Biological sciences · Health sciences
- From keywords
- Biochemistry and cell biology · Medicine & Health · Plant biology
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.1569161 | 14 d ago | JSON v1 |
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| concepts[field].fos:biological-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:health-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · DataCite | vocabulary-mapper@1.0.0 | keywords['Medicine'] |
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