Data · dataset · 2024
Dnitrification-synC2
Listed in ScienceDB
On March 18, 2024, the synthetic microbial community was resuspended in low-carbon-source 2216E medium with 7% salinity to an OD600 of approximately 0.8.
Description
The culture was incubated at 25 °C in a biochemical incubator for 1 hour and then immediately centrifuged at 4,500 r/min for 10 minutes to collect the cell pellet. The centrifuge tube was rapidly frozen in liquid nitrogen, and the samples were preserved on dry ice and sent to Novogene Bioinformatics Institute (Novogene Bioinformatics, Beijing, China).
Metatranscriptomic sequencing was performed based on the NovaSeq 6000 sequencing system (library insert size of 350 bp, paired-end sequencing length of 150 bp), generating 10 Gb of data per sample.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.14408 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Ocean & Atmospheric Science · Social Science
- Inferred from text
- Environmental biotechnology 69% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.14408 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:4103 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |