Effect of artificial control system (hQSCS) on native transcriptome of S. erythraea during erythromycin fermentation

Here, we present a transcriptional profiling dataset of S. erythraea strain harboring the heterologous QS control system (hQSCS) during erythromycin fermentation. This dataset was generated to characterize the transcriptional response of host cells upon activation of the heterologous hQSCS.

Specifically, RNA sequencing was performed on strain without hQSCS (control) and hQSCS engineered strain (treatment), both cultured in TSB medium for 72 h. Differential expression analysis of this dataset will help determine whether hQSCS exerts regulatory crosstalk with the native transcriptome of S. erythraea, which is essential for verifying the orthogonality of hQSCS. Collectively, this transcriptional dataset provides a valuable resource for investigating the orthogonality of hQSCS in S. erythraea and offers insights into the development of orthogonal control systems for other rare actinomycetes.