Sensitivity to copper and development of culturing and toxicity test procedures for the Antarctic terrestrial nematode Plectus murrayi

AAS_4100_Pmurrayi_Agar.xlsx Aqueous exposure Three solutions were tested to determine the optimum medium for P. murrayi in aqueous exposures. Survival was scored by the numbers of live and dead nematodes counted in each replicate. Hatching success was estimated as percent of eggs that hatched per replicate.

Hatched juvenile survival was expressed as percent survival relative to hatching success for each replicate. AAS_4100_Pmurrayi_Aqueous_solution_egg_hatch_juv_adult_surv.xlsx Sensitivity of Plectus murrayi to aqueous copper The sensitivity of hatched juveniles to Cu was estimated after a total of 28 d exposure (egg to hatched J2 juvenile). Two tests.

Survival was scored by the numbers of live and dead nematodes counted in each replicate. Hatching success was estimated as percent of eggs that hatched per replicate. Hatched juvenile survival was counted in one test and was expressed as percent survival relative to hatching success for each replicate.

AAS_4100_Pmurrayi_Cu_egg hatch_juv_survival.xlsx Juvenile survival in aqueous copper. Two tests. Survival was scored by the numbers of live and dead nematodes counted in each replicate.

AAS_4100_Pmurrayi_Cu_juv_survival.xlsx Aqueous copper concentrations in tests were measured using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) (Varian 720-ES). The measured dissolved Cu concentrations (µg/L) used in statistical analyses of sensitivity estimates was the geometric mean of initial and final concentrations for each treatment. Copper concentrations are given in the spreadsheets for tests with copper.