<p>Motif sequence and their width.</p>

A total of 15 <i>ITPK</i> genes have been identified in wheat (<i>T. aestivum</i>). The <i>TaITPK</i> genes were distributed on chromosome number 1, 4, 5 and 7 in all the three genomes A, B and D. Comparative phylogenetic analysis resolved four major groups, with wheat <i>ITPK</i> sequences represented in Groups I, III, and IV, whereas Group II contained no wheat sequence. The conserved domain and motif analysis showed the presence of Ins134_P3_kin domain at N-terminus and CPase_L_D2 in C-terminus of protein.

String based protein interaction analysis suggested <i>ITPK</i> genes interact with Inositol polyphosphate multi kinase (<i>IPK1</i>) and Inositol-pentakisphosphate 2 kinase (<i>IPK2</i>) which were also involve in synthesis of phyti acid. Furthermore, in-silico expression analysis of <i>ITPK</i> genes revealed their preferential tissue-specific and growth stage expression, especially after anthesis of wheat. Preliminary qRT-PCR profiling of selected <i>TaITPK</i> genes revealed treatment-dependent relative expression patterns under FeSO₄ and ZnSO₄ supplementation.

The current study has identified 15 <i>ITPK</i> genes present in hexaploid wheat genome. Their evolutionary, structural, and expression analyses provide a foundation for further functional characterization of this gene family in wheat.</p></div>