<p>Characterization of Nuclear Protein Extracts and Histone Abundance During the <i>T. cruzi</i> Cell Cycle.</p>

DNA was stained with DAPI (blue), and the flagellum was labeled with the anti-flagellum monoclonal antibody mAb 2F6 (green). Brightfield images are shown on the right.

N, nucleus; K, kinetoplast. <b>(C)</b> SDS-PAGE (15%) of epimastigote and nuclear extracts stained with Coomassie blue, showing enrichment of histones (~15 kDa) in the nuclear fraction. <b>(D)</b> Representative western blot assay showing the presence of nuclear marker protein (anti-H3) and the absence of ant-HSP70 (endoplasmatic reticulum), anti-aldolase (glycosomal) in the nuclear fraction and whole cell lysate. <b>(E)</b> Venn diagram showing the overlap of proteins identified in the nuclear proteome across the three cell cycle phases analyzed. <b>(F)</b> Venn diagram comparing proteins identified in our nuclear cell cycle proteome (CC_PN, green) with those previously reported in the <i>T. cruzi</i> nuclear proteome (PN, orange) (1) and chromatin-associated proteome (Chr_P, blue) (2).</p>