Dataset of SARS-CoV-2 wastewater data from Knivsta, Sweden

This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Knivsta.<div><br>The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of Agricultural Sciences) and her research groups in collaboration with Uppsala Vatten. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.<div><br></div><div>The viral content is concentrated according to the protocol from Ahmed et al. <a href="doi.org/10.1016/j.scitotenv.2020.139960" target="_blank">doi.org/10.1016/j.scitotenv.2020.139960</a></div><div>according to the modified electronegative filtration method.

Viral RNA is extracted and SARS-CoV-2 gene copy nr quantified using R-qPCR. Recovery efficiency is measured using Bovine coronavirus (BCoV) as a virus surrogate. Pepper mild mottle virus (PPMoV) is used to correct for variations in water size and flow.