<p>Effects of AZP2006 on TH(+) primary mesencephalic neurons cultured without microglia (A-G) or WT SH-SY5Y cells (H) and injured by α-syn PFF preparation for 96 h (primary cultures) or 72 h (SH-SY5Y cells).</p>

<p>ICC analysis of cultures shows the total number of TH(+) neurons (A), the length of their neurite network (B), the area of cytoplasmic α-syn staining per neuron (C), the area of LAMP2(+) staining per TH(+) neuron (D), the α-syn accumulation in lysosomes as α-syn/LAMP2 colocalization area (E), autophagosomes as LC3b(+) vesicles per TH(+) neuron (F), autolysosomes as LC3b(+) vesicles colocalized with LAMP2 area in TH(+) neurons (G), and functional lysosomes expressed as area of PhenoVue 577(+) staining per SH-SY5Y cell (H), expressed as % of control (CTR).

Raw values for CTR (i.e., 100%); sum TH(+) neurons per well, i.e., per biological replicate = 32 ± 1.3; neurite network = 1054 ± 60.6 µm; α-syn area/TH(+) neuron = 25.2 ± 2.8 µm²; LAMP2 area/TH(+) neurons = 17.9 ± 1.5 µm²; α-syn area/LAMP2/TH(+) neuron = 4.4 ± 0.3 µm²; LC3b area/TH(+) neurons = 27 ± 1.7 µm²; LAMP2/LC3b area/TH(+) neurons = 7.8 ± 0.5 µm²; PhenoVue 577(+) staining/SH-SY5Y cell = 67 ± 3.5 µm².