Exploration of the structure-function relationships of two novel antimicrobial peptides from amphibian skin secretion, through rational design and in vitro studies

Two novel AMPs, t-DPH1 and B1AW were first isolated from frog skin secretion in this project. A series of bioassays were conducted to evaluate their bioactivities, and the natural AMPs were used as templates to explore the structure-function relationships and optimise their bioactivities.<br>Through a series of bioassays, the bioactivities of two novel peptides were evaluated.

Through rational design, the structure-function relationships of these two peptides were explored. It was proved that the three conserved features of the dermaseptin family were indispensable to the antimicrobial activity of t-DPH1. The cationicity-enhanced analogue, t-DPH1-K was regarded as a potential drug candidate with dual effect and low possibility to induce drug resistance.