Thermus thermophilus TnSeq

The transposition library generated in vitro was initialy transformed in a PrimPol mutant HB27 derivative (ppol), which shows ~2 log fold transformation efficiency. This allowed the generation of the generation of a large library of insertion mutants (“Mother”), which was later transferred to a wild type HB27 strain (“Daughter”).

Both libraries were sequenced straightforward and after several culture passages. See the manuscript for more details. This dataset contains the raw data of the sequencing libraries as well as the GitHub repository containing the scrips and reports from the analysis.