Omics · study · 2026
Effect of Alendronate on Survival and Bone Properties in Nothobranchius furzeri: Insights from a Model of Accelerated Aging
Listed in NCBI GEO
Alendronate, a nitrogen containing bisphosphonate, effectively inhibits osteoclastic bone resorption and is considered as first line treatment of osteoporosis and Paget´s disease.
Description
Additionally, several studies suggest a beneficial effect on mortality that goes beyond life-extending effects attributed to the well-established fracture risk reduction. However, mechanisms of the mortality reducing effect of bisphosphonates are unclear.
The turquoise killifish Nothobranchius furzeri (N.furzeri), a well-known model of accelerated aging, exhibits an extremely short life cycle and thus could represent a suitable model for survival studies. The aim of this study was to test the effect of alendronate on the life span and bone properties of N.furzeri. 89 female fish were divided at the age of 16 weeks into a treatment (10µM alendronate, n=44) and a control (n=45) group and monitored for survival.
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Vertebral bodies of deceased fish were examined by micro-CT and bone histomorphometry. RNA sequencing and gene expression analysis was done from isolated tissues of heart, spleen, liver, muscle and kidney. Comparing both groups, the alendronate group showed a significantly higher survival (p=0.0070).
Micro-CT evaluation showed significantly higher values for bone (+2%; p=0.0013) and tissue mineral density (+3%; p=0.0159) in the control group. Bone histomorphometry displayed significant higher values for number of osteoblasts/bone perimeter (p=0.0340) and number of osteoblasts (p=0.0335) in the alendronate group. RNA sequencing revealed differences in both gene expression and biological function with alendronate treatment.
Looking at the gene expression levels, we determined a reduced ZNF703 expression in the alendronate group. Our results indicate that alendronate has a positive effect on the survival of N.furzeri and alters bone mineralization. Furthermore, it effects several non-bone related pathways.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313857 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313857 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1381684 ncbi.nlm.nih.gov/bioproject/PRJNA1381684 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · Heart 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313857 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |