Omics · study · 2026
The UAP2-BRR2b Module Confers Cold Tolerance by Safeguarding Splicing Fidelity in Arabidopsis
Listed in NCBI GEO
Plants adapt to cold stress through transcriptomic reprogramming, yet how the core spliceosome maintains splicing fidelity under low temperature remains poorly understood.
Description
Here, we identify an U4/U6.U5 tri-snRNP-associated protein 2 (AtUAP2) as a cold-specific regulator of pre-mRNA splicing in Arabidopsis. Under normal conditions (22°C), the uap2, brr2b, and uap2 brr2b mutants exhibit no developmental anomalies.
Upon cold stress, however, uap2 displays severe and specific cold sensitivity. Transcriptome-wide analysis revealed that UAP2 deficiency leads to widespread mis-splicing under low temperature. Expressing correctly spliced AtGRP7 and AtLOS2 cDNAs in uap2 partially rescues its cold sensitivity, confirming both as downstream effectors.
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Mechanistically, UAP2 physically interacts with the U5 RNA helicase BRR2b. While the brr2b mutant displays cold sensitivity similar to uap2, the uap2 brr2b double mutant exhibits an even more pronounced cold-sensitive phenotype. Crucially, this genetic synergy and cold-specific vulnerability demonstrate that the UAP2-BRR2b complex operates as a dedicated functional module required to sustain spliceosomal efficiency under cold stress, thereby providing a key mechanistic link between core splicing machinery dynamics and plant cold tolerance.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346033 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346033 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1522790 ncbi.nlm.nih.gov/bioproject/PRJNA1522790 ↗
project · from NCBI GEO
Topics
- Stated by source
- Arabidopsis thaliana · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Agricultural biotechnology 69%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346033 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3001 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:3702 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |