Bulk RNAseq of K7M2 osteosarcoma cells in engineered bone marrow treated with Doxorubicin

We interrogate the influence of the anatomical implantation site on the quality of generated tissue, test ex vivo stability under normoxic (5% O2) and standard (21% O2) culture conditions, and culture OS cells within these eBM constructs. We show that eBM accurately and stably recapitulates the composition of native bone marrow. OS cells exhibit differential behavior dependent on metastatic potential when cultured in eBM, thus closely mimicking in vivo conditions.

Furthermore, we highlight the clinical applicability of eBM as a drug-screening platform through doxorubicin treatment of OS cultured in monolayer, Matrigel, or eBM, and we show that eBM confers a protective effect on OS cells that parallel clinical responses. Combined, this work presents eBM as a cellular construct that mimics the complex bone marrow environment that may be useful for mechanistic bone cancer research and drug screening.