Not the same: phylogenetic relationships and ecological niche comparisons between two different forms of Aglaoctenus lagotis from Argentina and Uruguay

From those we obtained three mitochondrial markers including the 5’ half of cytochrome c oxidase subunit I (cox1), the 3’ half of the 16S rRNA ribosomal subunit plus the complete tRNA-Leu plus 5’ half of the NADH dehydrogenase subunit I (16S+L1+nad1), and a partial fragment of the small ribosomal unit (12S).

Additionally, we sequenced the nuclear intron of the gene encoding translation initiation factor 5A (tif5A). The sequences of the 12S, 16S+L1+nad1 and tif5A gene fragments were aligned using the online version of MAFFT v7 using the Q-ins-i algorithm. The alignment of the partial fragment of cox1 sequences was trivial since no insertions/deletions (indels) were observed.