An N-Phosphoproteomics Dataset of Human Hippocampal Neurons

This study presents an N-phosphorylation proteome dataset generated from human hippocampal neuronal cells (HPPNCs) transfected with the Tau-P301L mutation, an in vitro cultured cell model, a variant associated with tauopathy.

N-phosphopeptides were enriched under neutral conditions using affinity peptide-functionalized magnetic beads (MB@PA) and analyzed by high-resolution LC-MS/MS. Raw data were processed using MaxQuant for label-free quantification, with contaminants and reverse hits excluded. The dataset comprises 6,064 N-phosphorylation sites mapped to 3,372 proteins, including 1,286 pHis, 2,462 pLys, and 2,316 pArg sites, of which 2,383 met high-confidence criteria with filtered using localization probability ≥ 0.75, score ≥ 40, and score difference ≥ 5.