Data · dataset · 2015
Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, Eisenia andrei , using expressed sequence tags
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The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions.
Description
To gain insight into the expression profiles of coelomic cells of the earthworm, Eisenia andrei , we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs.
From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of E. andrei were upregulated after the injection of Bacillus subtilis bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
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Where it is published
- Repository landing page tandf.figshare.com/articles/dataset/Gene_expression_profiling_of_coelomic_cells_a… ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.1267559.v3 ↗
DOI / persistent id · from DataCite
Documentation and papers
- Creative Commons Attribution 4.0 International creativecommons.org/licenses/by/4.0/legalcode ↗
license · from DataCite
- IsSupplementTo 10.1080/09168451.2014.988677 doi.org/10.1080/09168451.2014.988677 ↗
publication · from DataCite
Catalogue records · 2
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1267559.v3 ↗
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- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1267559.v3 ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Biological sciences · Clinical medicine
- From keywords
- Ecology · Evolutionary biology · Immunology · Life Sciences
Related
- Has versionGene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, Eisenia andrei , using expressed sequence tags
- Possibly the same asGene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, Eisenia andrei , using expressed sequence tags
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.1267559.v3 | 11 d ago | JSON v1 |
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| concepts[field].local:field:life-sciences | mapping · DataCite | vocabulary-mapper@1.0.0 | keywords['Biological Sciences'] |
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