Data · dataset · 2022
Dataset for bacterial community and metagenome in soils affected by straw, biochar and ash
Listed in ScienceDB
Three treatments of soil experiment were set up.
Description
The straw and the biochar and ash made from equal amount of straw were amended into soils, respectively. We aimed to investigate the soil bacteria community and metagenome driven by different forms of the straws. (1)After DNA extraction, Primers 505F (5'-GTGYCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT-3') were used to amplify 16 s rRNA genes V3-V4.
Purified product were sent to biological company for sequencing. Data quality control and split were conducted to obtain the clean data (1 fastq file per sample).(2) High quality DNA samples were sent to biological company for the construction of 350 bp gene library, and then paired end 2 × 150bp sequencing was performed on an Illumina HiSeq X Ten platform. Approximately 12 Gb of raw data were obtained for each sample.
Read the rest (1 more)
After quality control, two read files, fq1 and fq2, were given for each sample.
Links
Where it is published
- DOI doi.org/10.11922/sciencedb.o00113.00005 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.11922%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Evolutionary biology 71% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.11922/sciencedb.o00113.00005 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3104 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (71%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |