Open-source hardware solutions for longitudinal recordings of in vitro neuronal networks on MEAs based on Inkube: Manufacturing files for inkudock and a water compartment cell culture lid

This repository contains manufacturing files (CAD designs and PCB layouts) for two open-source hardware components of the inkube electrophysiology and culturing platform: inkudock and a water compartment cell culture lid. inkudock is a modular incubator comprising a central reservoir with resistive heating, CO₂ supply, and circulation, and up to four attachable chambers, each providing independent Peltier-based temperature control of the MEA recording unit.

The water compartment lid sits atop the cell culture and eliminates evaporation in non-humidified environments whilst maintaining gas exchange. The system supports both standard passive MEAs via the included open-source closed-loop electrophysiology, and commercial CMOS-based high-density MEA systems. Both components are designed for longitudinal multi-electrode array (MEA) recordings.