<p>Glycerol induces the CSP and glycation in <i>B. burgdorferi.</i></p>

<p>(A) <i>B. burgdorferi</i> were grown to ~7 × 10⁷ cells ml^-1 before collection by centrifugation (0h) and resuspension in RPMI (-), RPMI with 10, 30 or 100 µM glycerol or 10, 30 or 100 µM methylglyoxal (MG) and incubation for 24 h at 35°C. Equal amounts of whole cell lysates were separated by SDS-PAGE and visualized by immunoblot analyses with anti-MG antibodies to detect protein glycation and anti-FlaB antibodies as a loading control.

(B) Cells were grown as in panel A but were incubated in RPMI + 0.4% neopeptone or BSK without bovine serum albumin or rabbit serum (BSK -BSA -RS) for 24 h at 35°C. (C) Cells were grown as in panel A but were incubated in RPMI + 100 µM glycerol alone (-) or containing BSA (+BSA), RS (+RS) or both (+BSA + RS) for 24 h at 35°C. Also, cells were incubated in RPMI containing 500 µM mannose, maltose, trehalose or <i>N</i>-acetyl glucosamine (GlcNAc) for 24 h at 35°C. Cell lysates were analyzed by immunoblot as described in panel A.</p>