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Data · dataset · 2013

Raw data for Figure 3B: Mesoangioblasts and HIDEMs suppress T cell proliferation in a dose dependent manner

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CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at decreasing ratios (HIDEM/mesoangioblast:PBMC).

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On day 6 cells were harvested and stained with anti-CD3 antibody and 7AAD, and analysed by flow cytometry. CFSE dilution was analysed on gated CD3+ 7AAD- cells.

The percentage of CD3+CFSE dividing cells was calculated for each group and compared to the positive control (P+B), followed by plotting against HIDEM/mesoangioblast:PBMC ratios. Experiments were carried out in duplicates. n=2

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DataCite10.6084/m9.figshare.10672514 d agoJSON v1
DataCite10.6084/m9.figshare.106725.v114 d agoJSON v1
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concepts[disease].local:disease:cancermapping · DataCitevocabulary-mapper@1.0.0keywords['Cancer']
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