Data · dataset · 2026
Identification and functional study of a bioactive peptide QUB-1962 from the skin secretion of the yellow-bellied toad (Bombina variegata)
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32629974.v1
Because of the continuous emergence of drug-resistant strains, more and more antibiotics have lost their due role.
Description
The antimicrobial peptide has become the most promising substance in the next class of antimicrobial agents. QUB-1962 is a cationic antimicrobial peptide isolated from the skin secretions of Bombina variegata.
Using the "shotgun" cloning technique, a novel full-length peptide precursor of the bombinin peptide was cloned from the cDNA library of skin secretion from Bombina variegata. The speculative peptide was identified as QUB-1962 (IIGPVLGMVGNALGGLLKKI-NH2). And the crude peptide of QUB-1962 was obtained by solid-phase synthesis.
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Then, QUB-1962 was identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. In addition, QUB-1962 exhibited a broad-spectrum antimicrobial effect against Gram-positive bacteria Staphylococcus aureus (S. aureus), Gram-negative bacteria Escherichia coli (E. coli), and fungus Candida albicans (C. albicans) and has relatively weak haemolytic activity (HC50 is 691.6 μl). The anti-cancer activity assay showed that the peptide had a significant anti-cancer effect (IC50: 3.471×10-5 M) on the tested human cancer cell glioblastoma U251 MG.
According to the data of these biological activities, QUB-1962 has a wide range of biological activities, including antimicrobial and anticancer activities. At the same time, it could be a good candidate for the development of new anti-cancer and antimicrobial infection drugs.
Links
Where it is published
- DOI doi.org/10.17034/32629974.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 4 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32629974 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32629974 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32629974 | 12 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32629974 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |