Dataset for: Selection and evaluation of at least ten advanced clones of the LBHT x LTVR population with low glycoalkaloid content in contrasting environments, with high resistance to late blight, high tuber yield, heat tolerance and adapted to mid and highlands in Huamachuco

Total glycoalkaloids analysis was performed using the method described by Burgos et al. (2014) in which the extraction of glycoalkaloids was executed using methanol and chloroform prior to concentration at 60 ° C in a rotary evaporator. The extract was transferred to 2% acetic acid solution and then purified using ammonium hydroxide at 85 ° C and ultracentrifugation at 27,000 rpm. The pellet was reacted with 85% orthophosphoric acid and read at 408 nm in a spectrophotometer.

The determination of total glycoalkaloids was performed against a standard curve of α-chaconine as a reference. This parameter was used as a criterion to select the clones to be further evaluated in the next season. Only clones with a total glycoalkaloid concentration below the safety limit for human consumption (20 mg / 100 g fresh weight) were selected.