Data · dataset · 2023
Transcriptome of Arabidopsis shoot apical meristem of Col, bdg1-4 and lacs2-4
Listed in ScienceDB
To analyze the dynamic expression of cutin genes regulated by Arabidopsis thaliana seedlings under light and dark, transcriptional analysis of cotyledons and SAM including apical hook of Arabidopsis thaliana seedlings was carried out.
Description
After germination, the seedlings were vernalized at 4 °for 2 days, cultured in 22 °dark for 4 days, and then cultured for 6 hours in dark(DCol, Dbdg1, Dlacs2) and under light for 6 hours(LCol, Lbdg1, Llacs2) respectively.
The samples were collected at the same time for transcriptomic sequencing. Each sample has three biological repeats. Total RNA was isolated with RNAiso Plus (9109, TaKaRa).
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Illumina sequencing libraries were generated using the standard protocol. The paired-end sequencing libraries were sequenced on an Illumina NovaSeq 6000 sequencer.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.14327 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Plant biology 71% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.14327 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3108 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (71%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |