Additional file 2 of Development and technical evaluation of a reusable 3D-printed cytospin funnel for enlarged cytospin generation

Supplementary Material 2: Macroscopic and microscopic evaluation of cell monolayers‘ qualitative area and cell density achieved by cytocentrifugation using a commercially available funnel and the novel 3D-printed cytospin funnel: A, B) microscopic cell density and distribution achieved with a conventional funnel and a sample volume of 250 µl (estimated practical cell-loading capacity of 100,000 to 200,000 cells under the tested conditions) and a magnification of 10x and 20x; C, D) microscopic cell density and distribution achieved with the novel funnel prototype and a sample volume of 450 µl (estimated practical cell-loading capacity of 400,000 to 800,000 cells under the tested conditions) and a magnification of 10x and 20x; E) exemplary macroscopic cell monolayer areas achieved with the 3D-printed funnel (top row) and the commercial funnel (bottom row).