Transcriptomic profiling of diabetic wound tissues treated with multifunctional microneedles integrating spermidine- and TGF-β3-regulated dental pulp stem cell spheroids

In this study, RNA sequencing was performed to investigate transcriptomic changes in diabetic wound tissues following treatment with multifunctional microneedles (DST-MNs) integrating spermidine- and TGF-β3-regulated dental pulp stem cell spheroids.

Full-thickness diabetic wounds were established in rats, and wound tissues from untreated diabetic wound animals and DST-MNs-treated animals were collected for transcriptome profiling. Differentially expressed genes and enriched biological pathways were analyzed to identify molecular mechanisms associated with DST-MNs-mediated wound repair. These data provide insights into transcriptional regulation involved in inflammatory modulation, extracellular matrix remodeling, angiogenesis, and diabetic wound regeneration.