Data · dataset · 2019
Supplemental data and data sets for: Extracellular Na+ and Orai1 contribute to endothelial wound healing (Xu et al)
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Endothelial migration is necessary for wound healing and angiogenesis.
Description
Na + has been incriminated as an important cellular signal mediating migration, although the ion channels regulating Na + permeation that contribute to migration remain poorly understood. We have shown that Orai1 suppresses both constitutive Na + leak and Na + entry through store-operated calcium entry channels.
Here, we examine the independent and combined contributions of extracellular Na + and Orai1 to pulmonary artery endothelial cell (PAEC) wound healing. We found that PAEC monolayers migrated into wound areas and achieved confluence within 24 hours post-scratch. There was a fast healing phase within 5 hours, followed by a slow-healing phase between 5 and 24 hours.
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Na + depletion reduced migration speed by at least 50%. Fewer cells at the migration front formed prominent lamellipodia. Some cells at the leading edge gradually detached from the monolayer.
Reduction of Orai1 decreased wound closure speed by 25% within 6 hours. This Orai1-dependence was absent in Na + -depleted media and after 6 hours. In a non-scratch cell migration assay, the gap areas were partially sealed within 24 hours.
Migration speed was reduced by 75% when extracellular Na + was replaced by choline. Orai1 did not contribute to non-scratch cell migration, but extracellular Na + did. Overall, extracellular Na + and Orai1 together acutely regulate endothelial wound healing.
Extracellular Na + continuously contributes to wound healing between 6 and 24 hours. Extracellular Na + but not Orai1 contributes to cell migration in the absence of injury. Orai1 may function as an injury sensor to acutely stimulate fast wound healing.
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- Repository landing page figshare.com/articles/Supplemental_data_and_data_sets_for_Extracellular_Na_… ↗
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- DOI doi.org/10.6084/m9.figshare.7731161.v1 ↗
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- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.7731161.v1 ↗
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Topics
- Stated by source
- Basic medicine
Related
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.7731161.v1 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| byte_size | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:basic-medicine | source · DataCite | connector:datacite@1.0.0 | |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| updated_date | source · DataCite | connector:datacite@1.0.0 |