Astrocytic prostaglandin transporter SLCO2A1 protects blood-brain barrier integrity following ischemic stroke

Co., Ltd., Wuhan, China), according to the manufacturer's instructions. The enriched libraries were quantified and sequenced on a DNBSEQ-T7 platform (MGI) using the PE150 sequencing mode to generate paired-end reads.

Briefly, mRNA was enriched and cleaved into short fragments using a fragmentation buffer, followed by reverse transcription into cDNA using random primers. The cDNA fragments were purified and end repaired, and a poly(A) tail was added, followed by ligation with Illumina sequencing adapters. The ligation products were then size selected by agarose gel electrophoresis, amplified by PCR, and sequenced using the DNBSEQ T7.