Excel · study · 2026
Loss of IL10RA rewires epithelial immune responses to intracellular Staphylococcus aureus small colony variants in bovine mammary epithelial cells
Listed in NCBI GEO
Staphylococcus aureus small colony variants (SCVs) are a persistence-adapted phenotype associated with chronic and recurrent bovine mastitis.
Description
The interleukin-10 receptor ⍺ (IL10RA) is a key regulator of anti-inflammatory signalling in the mammary gland, yet its role in governing transcriptional responses to intracellular staphylococcal challenge remains unknown. Using CRISPR/Cas9-generated IL10RA knockout (KO) MAC-T bovine mammary epithelial cells (MECs) and RNA sequencing, we compared transcriptional responses to infection with S. aureus SCV Heba3231 and its isogenic parental strain (PS).
The wild-type (WT) MAC-T cells mounted fundamentally divergent responses to the PS (8 DEGs) and the SCV (461 DEGs), with no transcriptional overlap, indicating strain-specific engagement of distinct molecular programmes. SCV infection of WT cells up-regulated lipid and sterol biosynthetic pathways, and suppressed genes associated with epithelial barrier integrity, including S100A8, S100A9, TGM3 and KRTDAP. IL10RA disruption dramatically amplified transcriptional dysregulation.
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The IL10RA-KO cells infected with the PS yielded 619 DEGs (77.4-fold increase over WT-PS), while IL10RA-KO cells infected with SCV yielded 1,297 DEGs (2.8-fold increase over WT-SCV). A moderate core of IL10RA-regulated genes (~32–36%) was shared across infection conditions. IL10RA loss derepressed pro-inflammatory cytokine pathways (TNF, IL-17), enhanced pro-apoptotic programmes during SCV infection, and suppressed phagosomal maturation and lipid metabolic gene programmes.
Novel candidate genes, including CD79B, WFDC2, AMN and LY6E, were identified as potential biomarkers of IL10RA-dependent mammary immune signalling. These findings establish IL10RA as a broad transcriptional homeostasis regulator in bovine mammary epithelial cells during intracellular staphylococcal infection.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334081 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334081 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1473692 ncbi.nlm.nih.gov/bioproject/PRJNA1473692 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE334081 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |