DNA methylation signatures predict cytogenetic subtype and outcome in pediatric acute myeloid leukemia (AML)

This dataset contains genome-wide DNA methylation data generated from 142 pediatric acute myeloid leukemia (AML) samples originating from bone marrow or peripheral blood samples taken at AML diagnosis (N=123) or relapse (N=19). Further details regarding the samples are available in Supplementary Table S1 from Krali and Palle et. al., 2021 (doi.org/10.3390/genes12060895). Genome-wide DNA methylation was analyzed at the SNP&SEQ Technology Platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden. 200ng of bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using the standard protocol from Illumina (iScan SQ instrument).

This metadata record contains information about the raw idat files generated from the Infinium DNA methylation arrays. The Methylprep Python library was used to generate and normalize the beta-value matrix (pypi.org/project/methylprep/1.3.3/). The raw idat files along with a samplesheet, processed beta-value matrix, annotation file for CpG annotation, and signal intensities matrix will be made available upon request.