<p>Curli induced cytokine responses in NZBxW/F1 mice.</p>

Periodic serum samples were collected 1 week before starting injections and 24 hours after the last injection, weekly for (<b>A</b>) 4 weeks or (<b>B</b>) 7 weeks. <b>(C-D)</b> Weight was tracked over time, normalized to the starting weight 1 week before treatment (<b>C</b>, N = 16; <b>D</b>, N = 19 Individual late-disease NZBxW/F1 mice that rapidly lost weight and had to be euthanized are shown separately.) <b>(E)</b> Late-disease NZBWxW/F1 mice were euthanized if 20% weight was lost or predetermined humane endpoints based on pain signs were reached; survival was tracked over time (N = 29, 3 replicate experiments). <b>(F-H)</b> Multiplex MSD U-plex ELISA detected <b>(F)</b> IL-6, <b>(G)</b> TNFα, and <b>(H)</b> IL-10 in serum 24 hours after the first curli injection in late-disease NZBxW/F1 mice; dotted line indicates lower detection limit of the assay.

Statistics: <b>(C)</b> Two-way ANOVA with Tukey’s multiple comparisons, <b>(D)</b> Mixed-effects model with Sidak’s multiple comparisons, (<b>C-D</b>) comparisons within legend indicating overall column factor, <b>(E)</b> Log-rank test, <b>(F-H)</b> Unpaired t-test (*p < 0.05, **p < 0.01, ***p < 0.001).</p>