Data from "Fishing in troubled waters: Revealing genomic signatures of local adaptation in response to freshwater pollutants in two macroinvertebrates"

Augustus was used for ab initio predictions. For the homology-based approach, protein datasets from related species (Apis mellifera, Nasonia vitripennis, Drosophila melanogaster, Anopheles gambiae, Plutella xylostella, Bombyx mori, Tribolium castaneum, Pediculus humanus, Acyrthosiphon pisum, Daphnia pulex, Caenorhabditis elegans and Homo sapiens) were aligned to the draft genome assemblies using BLASTp (cutoff: 10-5), and gene models were generated by GeneWise.

A consensus gene set that merged ab initio and homology-based predictions was created using GLEAN. The three resulting data files contain a table with summary information per predicted gene (Glossosoma_conformis.gff), the predicted mRNA sequences (Glossosoma_conformis.cds) and the predicted protein sequences (Glossosoma_conformis.pep). Functional annotation: Gene functions were assigned to the Geneset of the Glossosoma conformis draft genome using BLASTp (cutoff: 10-5) against KEGG (release 58), non-redundant protein sequences (NCBI release 20150222), Swiss-Prot, and TrEMBL (Uniprot release 201203).