A global regulatory atlas of Streptomyces reveals conserved and diversified transcriptional networks across actinomycetes

Here, we apply DNA affinity purification sequencing (DAP-seq) to 789 31 predicted TFs of Streptomyces coelicolor, generating genome-wide binding maps for 393 32 regulators and expanding the experimentally supported regulome from ~8% to ~50%. 33 Integration with ChIP-seq reveals pleiotropic regulators and hierarchical network architecture 34 linking primary metabolism, development and biosynthetic gene clusters (BGCs).

This GEO data set include 11 ChIP-seq results discussed in our manuscript