Impact of Solids Residence Time on Nutrient and Community Structure Dynamics of Algal-Bacterial Wastewater Treatment Systems

Specifically, the objective of this work was to study the effect of SRT on microbial community structure in mixed algal-bacterial wastewater treatment systems. Wastewater-fed reactors were operated in duplicate at distinct SRTs (5, 10, and 15 days) with diel lighting until long-term steady-state operation was achieved (>6 SRTs).

High-throughput sequencing was used to examine the microbial diversity, including bacteria and eukaryotes, over time. This project contains script files for analyzing data using mothur (scripts start with a fasta input file, following the "make.contigs" command in mothur to merge individual fastq files).