Raw data microbiota development Panda Study (part 2)

It was demonstrated that profiles are reproducible and that log2 transformation enables both the identification of slight variations between related profiles, as well as correlation analysis of large sets of profiles. Is-pro does not generate sequence data in a traditional sense. This means IS-pro cannot achieve the same level of detail as next-generation sequencing techniques, although sequence data confirmed specificity of IS-pro in 15 common peaks, underlining the validity of the technique.

Each sample is represented by a microbial profile, consisting of color-labeled peaks. Each peak is characterized by a specific IS fragment (measured in number of nucleotides) and a color related to a specific phylum group. The intensity of peaks reflects the quantity of PCR product (measured in relative fluorescence units (RFU)).

Each peak is designated as an operational taxonomic unit (OTU) and its corresponding intensity as abundance. Intensity values are log2 transformed in order to compact the range of variation in peak heights, to reduce the dominance of abundant peaks and to include less abundant species of the microbiota in downstream analyses. Here we share the raw data of the IS-pro analysis (part 2).