Data · dataset · 2026
Proteomics-Guided Discovery of Candidate Enzymes for Degradation of a Rare Pyruvylated Glycosaminogalactan by a Newly Isolated Luteolibacter Strain
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Bacterial exopolysaccharides (EPS) display extensive structural diversity arising from variation in monosaccharide composition, glycosidic linkages, and non-carbohydrate modifications.
Description
However, the enzymes responsible for degrading many EPS structures remain unknown, reflecting the limited coverage of current carbohydrate-active enzyme (CAZyme) families. Here, we investigated the microbial degradation of EPS<sub><i>Lp</i></sub>, a high-molecular-mass glycosaminogalactan from Lactiplantibacillus pentosus KW1 containing the uncommon GalNAcβ1 → 2(4,6-<i>O</i>-<i>R</i>-pyruvyl)Gal motif.
Enrichment cultivation with EPS<sub><i>Lp</i></sub> as the main carbon source led to the isolation of <i>Luteolibacter</i> sp. VK1, a novel strain capable of utilizing this biopolymer. Size-exclusion chromatography and MALDI-TOF mass spectrometry showed that VK1 depolymerizes EPS<sub><i>Lp</i></sub> into defined oligosaccharides, consistent with endo-acting cleavage followed by further processing.
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By combining genomics, AlphaFold-based structural annotation and comparative proteomics, we identified candidate CAZymes potentially involved in EPS<sub><i>Lp</i></sub> catabolism. Notably, a secreted GH123 protein and a distantly related GH123-like protein were strongly associated with EPS<sub><i>Lp</i></sub>-dependent growth, suggesting that this enzyme family, or related structural homologues, may contribute to extracellular EPS<sub><i>Lp</i></sub> depolymerization.
Together, these findings support a candidate pathway for degradation of a structurally complex, pyruvylated bacterial glycan and highlight potentially novel EPS-active enzymes whose proposed roles, predicted through bioinformatic analyses, await experimental validation.
Links
Where it is published
- DOI doi.org/10.1021/acs.jproteome.6c00586.s001 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Chemistry · Earth & Environmental Science · Genetics · Life Sciences · Microbiology
- Inferred from text
- Mass spectrometry 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34025004 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:chemistry | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |