Excel · study · 2026
Integrated miRNA-mRNA Analysis Reveals Regulatory Mechanisms of Muscle Fiber Transformation Underlying Meat Quality Variation in Chicken
Listed in NCBI GEO
Chicken muscle fibers are classified as oxidative or glycolytic, with distinct metabolic properties that influence meat quality.
Description
However, the molecular mechanisms linking fiber type to quality traits remain unclear. This study compared the pectoralis major (PEM) and soleus (SOL) muscles of Guangyuan Grey chickens, revealing significant differences in fiber characteristics and meat quality.
Transcriptome analysis identified key pathways—MAPK, PPAR, and calcium signaling—associated with fiber-type variation. The transcription factor HOXC10, enriched in SOL, showed inverse expression to its predicted regulator, miR-6701-3p. Dual-luciferase assays confirmed HOXC10 as a direct target of miR-6701-3p.
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Functional studies in skeletal muscle satellite cells demonstrated that miR-6701-3p promotes proliferation, inhibits differentiation, and induces fast-twitch fiber formation, while HOXC10 facilitates slow-twitch fiber development. These findings reveal a novel miR-6701-3p/HOXC10 axis that modulates muscle fiber types and provides molecular targets for improving poultry meat quality.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299746 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299746 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1276426 ncbi.nlm.nih.gov/bioproject/PRJNA1276426 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Gallus gallus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE299746 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9031 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |