Evaluation of the antibacterial activity and mechanism of action of CPF-MW1

The study aimed to evaluate the antimicrobial activity and the mechanism of action of the frog-derived antimicrobial peptide CPF-MW1 against Gram-negative bacteria using E. coli ATCC 700928 as a model organism. The objectives of the study included the following:</p> <ol><li>Antibacterial activity determination (both anti-planktonic and anti-biofilm)</li><li>Peptide cytotoxicity evaluation both on human erythrocytes and HaCaT cell line.</li><li>Mechanism of action evaluation including time-kill kinetics, membrane permeabilisation and scanning electron microscopy.</li><li>Peptide stability in physiological conditions evaluated by pre-exposure to proteolytic enzyme (trypsin) and fetal calf serum.</li></ol> <p><br></p> <p>All data provided represents a set of three independent experiments performed in triplicate, unless stated otherwise.

Data was analyzed and processed using GraphPad prism 8 software. Each data point was calculated as an average of three biological repeats each consisting of three technical repeats generating 9 data points unless stated otherwise. All data was expressed as means ± standard error of the means (SEM).