Data · dataset · 2024
Proteomic analysis of injured and uninjured nerves
Listed in Teesside University Research Data Repository
Murine injured sciatic nerves were harvested from C57BL/6J mice at 24h post-transection.
Description
Uninjured nerves were set as control group. Nerve samples were frozen and then ground into fine and uniform powder in liquid nitrogen for protein extraction.
Then the samples were digested and fractionated. The data independent acquisition (DIA) mass spectrometry analysis was acquired by TimsTOF Pro2 mass spectrometer (Bruker) and nanoElute (Bruker). For data independent acquisition (DIA), 56 DIA windows were acquired and the collision energy was ramped linearly as a function of the mobility from 59 eV at 1/K0 = 1.6 Vs/cm2 to 20 eV at 1/K0 = 0.6 Vs/cm2.
Read the rest (1 more)
The MS/MS spectra were recorded from 100 to 1700 m/z. The default factory settings were used for the Spectronaut Pulsar™ 15.3.210906.50606 (Biognosys, Swiss) search and library generation.
Links
Where it is published
- DOI doi.org/10.17632/k79f8rn24m.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Mass spectrometry
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/k79f8rn24m.1 | 9 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Proteomics'] |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |