Data · dataset · 2025
Gut Microbiome Profiles in CB2R-Knockout and Wild-Type Mice With and Without Salmonella Infection
Listed in Teesside University Research Data Repository
This dataset examines how genetic deletion of cannabinoid receptor 2 (CB2R) impacts gut microbiome composition during Salmonella enterica serovar Typhimurium infection.
Description
CB2R is a G protein-coupled receptor involved in regulating immune responses and intestinal homeostasis. Its expression in immune cells and the gastrointestinal tract suggests a potential role in shaping host-microbiota interactions.
To investigate whether CB2R deficiency alters microbial dynamics in a way that influences susceptibility to infection, we compared CB2R knockout (CB2R-KO) mice with wild-type (WT) littermate controls in an oral Salmonella challenge model. Fecal samples were collected at two time points: pre-infection (baseline) and four days post-infection. Samples were subjected to shallow shotgun metagenomic sequencing (1×150 bp, Illumina).
Read the rest (2 more)
Raw reads were filtered using Vsearch to remove low-quality sequences and short fragments. Taxonomic classification was performed with Kraken2, and processed data were analyzed using R (phyloseq, ALDEx2, microbiome packages). Alpha diversity analysis showed no significant difference in species richness or evenness between genotypes.
However, CB2R-KO mice exhibited distinct shifts in microbial composition following infection. These findings suggest that CB2R contributes to maintaining a balanced gut microbial ecosystem and that its absence may promote dysbiosis during enteric infection, potentially increasing host susceptibility.
Links
Where it is published
- DOI doi.org/10.17632/7pn4pwj3td.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/7pn4pwj3td.1 | 8 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |