<p>Representative flow cytometric analysis of phagocytic capacity in neutrophils following co-incubation with zoospores, showing flow cytometry plots and histograms of gated neutrophils from healthy donors (A, B).</p>

PE-positive cells indicate neutrophils that had phagocytosed <i>P. insidiosum</i> zoospores. Neutrophils co-incubated with zoospores at 4°C were used as a negative control.

Quantification of the percentage of neutrophil phagocytosis after incubation with zoospores, compared with negative controls (E). The data are shown as the mean ± S.E.M., *, <i>p-value</i> = 0.0335 vs. MOI 1 of β-thalassemia patients, using the Kruskal-Wallis test (n = 6/group). Abbreviations are as follows: MOI 1, co-incubation of neutrophils isolated from either healthy individuals or β-thalassemia patients with <i>P. insidiosum</i> zoospores at a multiplicity of infection (MOI) of 1.</p>