Effects of Hedysari Radix before and after honey-processing paired with vinegar-processed Curcumae Rhizoma on apoptosis and autophagy of lung cancer cells-raw data

This study aims to compare the effects of honey processed Hedysari Radix-vinegar-processed Curcumae Rhizoma (HPHR-VPCR) and Hedysari Radix-vinegar-processed Curcumae Rhizoma (HR-VPCR) on apoptosis and autophagy in A549 cells and NCI-H1299 cells, and explore the mechanism of HPHR-VPCR inducing apoptosis and autophagy. Twenty-one SPF grade SD rats were randomly divided into three groups: control (Con) group, HR-VPCR group, and HPHR-VPCR group.

The dosage of each drug pair was 4.63 g/kg, and the drug containing serum was taken after continuous administration for seven days; Hoechst33258 staining was used to detect cell apoptosis morphology, and flow cytometry was used to detect cell apoptosis rate; The monodansylcadaverine (MDC) method was used to detect cellular autophagy; Transmission electron microscopy was used to detect autophagosomes and autolysosomes; Western blot was used to detect the expression of ubiquitin-binding protein P62 (P62), beclin1(BECN1), B-cell lymphoma-2 (BCL-2), and BCL-2 associated X protein (BAX) proteins.