Omics · study · 2026
Genome-wide identification of NAC transcription factors in Elymus sibiricus and functional characterization of EsANAC029 in light response
Listed in NCBI GEO
Elymus sibiricus L. is a perennial grass of high pastoral and ecological value, playing a critial role in to livestock production and ecological restoration on the Qinghai-Tibet Plateau.
Description
NAC transcription factors are crucial regulators of plant development and abiotic stress responses, yet they remain uncharacterized in E. sibiricus. Here, we report the first genome-wide analysis of the NAC family in this allohexaploid species, and identified 290 EsNAC genes.
These genes are classified into 14 subfamilies, 11 of which have undergone significant expansion mainly through segmental duplication events, suggesting a potential genetic basis for adaptation to plateau environments. We revealed a pronounced enrichment of light-responsive cis-elements in the promoters of these genes. Further analysis in Arabidopsis showed significant overlap between AtNAC and a set of light-repressed genes, strongly implicating the NAC family in light inhibition pathways.
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Notably, heterologous overexpression of EsANAC029 (an ortholog of Arabidopsis AtANAC029) in Arabidopsis recapitulated a clear light-inhibition phenotype, including chlorosis and dwarfism. Transcriptomic profiling indicated that EsANAC029 represses the expression of chlorophyll biosynthesis and photosynthesis-related genes, thereby reducing chlorophyll accumulation, impairing photosynthetic capacity, and ultimately leading to growth retardation.
In addition, EsANAC029 upregulates genes associated with defense-related pathways, linking light perception with stress adaptation. This work identifies EsANAC029 as a key negative regulator of photosynthesis, likely serving as an integrative node that connects light perception with stress adaptation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318634 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318634 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1419690 ncbi.nlm.nih.gov/bioproject/PRJNA1419690 ↗
project · from NCBI GEO
- PubMed 42226512 pubmed.ncbi.nlm.nih.gov/42226512 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Arabidopsis thaliana · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE318634 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:3702 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |