Data · dataset · 2026
<p>Grass carp NHRE3 inhibits ISG15-mediated antiviral function.</p>
Listed in ZivaHub
<p>(A) CIK cells co-expressing ISG15 and NHRE3 were infected with GCRV.
Description
CPE was assessed using crystal violet staining 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls.
(B) GCO cells co-expressing ISG15 and NHRE3 were infected with GCRV. The expression level of the GCRV VP5 protein was measured via western blot 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls.
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(C) GCO cells co-expressing ISG15 and NHRE3 were infected with GCRV. The expression levels of the GCRV S6, S10, and NS1 segments were assessed using qPCR 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls.
(D) GCO cells co-expressing ISG15 and different NHRE3 truncation mutants were infected with GCRV. The expression levels of the GCRV S6, S10, and NS1 segments were assessed using qPCR 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls.
(E) GCO cells co-expressing ISG15 and different NHRE3 truncation mutants were infected with GCRV. The expression level of the GCRV VP5 protein was measured via western blot 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls.
All experiments were repeated for at least three times with similar results.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014544.g006 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 1 source records, 17 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34026301 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
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| concepts[field].anzsrc:field:320211 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:440710 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:group:3103 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
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| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3108 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Plant Biology'] |
| concepts[field].anzsrc:group:3204 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |