Single-cell short- and long-read transcriptomic atlas of seven reproductive-axis tissues in male Baoshan pig

To characterize both gene-expression profiles and transcript isoform diversity across these tissues, single-cell short-read libraries were sequenced on an Illumina platform and single-cell long-read libraries were sequenced on an Oxford Nanopore platform.

Short-read data were processed with Cell Ranger, whereas long-read data were processed with FLAMES and further assessed for isoform structure. The dataset provides cell-level gene expression matrices, transcript/isoform-level quantification, isoform annotation files, and accompanying cell metadata. These data may facilitate studies of cellular heterogeneity and transcript isoform usage across the porcine reproductive axis.