whole-brain single-neuron resolution calcium response to discrete and continuous stimuli in larval zebrafish

They also both consist of 7 different types of stimuli: continuous, five different refreshing frequency (2.5Hz, 5Hz, 7.5Hz, 15Hz, 15-5Hz) and random stimulus. 15-5Hz means that along the square path, there are two invisible blocks in which the refreshing rate is down to 5Hz. One trial lasts for 17 s, consisting of 12 s of stimulus presentation and 5 s of interval. The stimuli are delivered randomly.

The difference of the second paradigm is that the square will joggle near its position at the frequency of continuous stimuli. So we controlled all stimuli to have the same local refreshing rate and the only difference is the global continuity. The first paradigm consists of three fish while the second paradigm has one fish.After we acquired the raw imaging data, we first performed a rigid registration to compensate for x-y plane drift.

Then we identify cell ROIs and calculated the dff trace of each neuron. The process is similar to this previous paper (Kawashima et al., 2016) The data we uploaded here are the .mat files after preprocessing, consisting of dff trace of every neuron and also files of stimulus information which can be read by a MATLAB script which we will provide if the request is granted.