<p>Viability of the LD spirochete <i>B. burgdorferi</i> in glycerol and MG.</p>

<p>Wild-type <i>B. burgdorferi</i> were grown in normal growth medium before shifting to RPMI medium alone or RPMI containing increasing concentrations of glycerol (A) or MG (B) for 24 h at 35°C. Cells were plated in semi-solid BSK before and after shifting to RPMI alone (0) or with increasing concentrations of glycerol or MG. (C) Wild-type (WT), <i>mgsA</i> mutant (∆<i>mgsA</i>) and <i>mgsA</i>-complemented (<i>mgsA</i>⁺) strains were grown as described in panel A, shifted to RPMI medium containing 100 µM glycerol for 24 h at 35 °C and then plated.

Values are the mean of three independent biological replicates expressed as % survival compared to cultures before shifting to RPMI. Error bars are the SE of the mean. Asterisks represent: