Data · dataset · 2026
Characterisation and analysis of QUB-1400: a peptide from the skin secretion of the Chinese frog, (Rana pleuraden)
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32640126.v1
Antimicrobial peptides (AMPs) have emerged as promising alternatives to conventional antibiotics due to their broad-spectrum antimicrobial activity and low likelihood of inducing resistance.
Description
This thesis focuses on a newly discovered AMP, QUB-1400, belonging to the pleurain family, which was isolated from the skin secretion of the Chinese southwestern frog, Rana pleuraden. Initially, the cDNA encoding the biosynthetic precursor of QUB-1400 was successfully cloned using 'shotgun' cloning techniques.
From this sequence, the structure of the mature peptide was deduced as FLGGLLSGIFKHL-NH2. Following this, the peptide was identified in the frog's skin secretion and synthesised for further investigation. QUB-1400 is a probable member of the pleurain-B AMP family.
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It underwent a series of bioactivity assays, including antimicrobial assays, a haemolysis assay, and an anti-proliferation assay. These assays aimed to assess the peptide's efficacy in combating microorganisms, its potential toxicity to mammalian cells, and its ability to inhibit cell proliferation.QUB-1400 demonstrated potent antimicrobial activity against various pathogens. Specifically, it displayed a low minimum inhibitory concentration (MIC) and mini-mum bactericidal concentration (MBC) against Staphylococcus aureus (MIC = 4 μM; MBC = 4 μM) and Escherichia coli (MIC = 4 μM; MBC = 8 μM), as well as Can-dida albicans (MIC = 16 μM; MBC = 32 μM).
Additionally, it exhibited an-ti-proliferative properties against the NCI-H838 lung cancer cell line, with an IC50 value of 21.29 μM. However, despite its promising bioactivity, the QUB-1400 pep-tide showed a relatively high half-maximal haemolysis concentration (HC50) of 50.12 μM. This could potentially limit its clinical utility and warrants further opti-misation to enhance its safety profile.<br><br><i>Thesis embargoed until 31 December 2029.</i>
Links
Where it is published
- DOI doi.org/10.17034/32640126.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32640126 | 11 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32640126 | 11 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32640126 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |