Omics · study · 2026
Protocol for scalable generation of uniform 3D porcine oviduct epithelial cell spheroids
Listed in NCBI GEO
While previous studies have reported in vitro formation of oviduct epithelial spheroid models in porcine species (sus scrofa), these approaches often rely on manual selection of the formed aggregates.
Description
Here, we establish a scalable and reproducible system for generating oviduct spheroids. We describe steps for the isolation of primary secretory and ciliated oviduct epithelial cells and their assembly into spheroids using the AggreWell platform.
This approach enables the mass production of uniformly sized spheroids ready for use in experiments such as sperm-oviduct co-incubation experiments.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335056 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335056 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1477182 ncbi.nlm.nih.gov/bioproject/PRJNA1477182 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335056 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |