Data · dataset · 2026
<p>Key enzymes of glycolysis and the TCA cycle exhibit nuclear activity.</p>
Listed in ZivaHub
<p><b>(A)</b>Activity of hexokinase (HK), citrate synthase (CS), and pyruvate kinase (PK), glycerol kinase (GK) and pyrroline-5-carboxylate dehydrogenase (P5CDH) were obtained using either nuclear and cytoplasmic extracts.
Description
The controls used here were not found in chromatin and nuclear proteomes. Reactions without the enzyme substrates were used to rule out residual activity.
Enzymatic activities were normalized with the total activities of parasite extract for each listed enzyme. Means ± SD of triplicate data (n = 3) are graphically represented. *p < 0.01; **p < 0.001; *** p < 0.0001. One-way ANOVA (Bonferroni’s Multiple Comparison Test). <b>(B)</b> KEGG map of the glycolysis and TCA cycle pathways. <b>(C)</b> Immunofluorescence analysis of hexokinase (HK), citrate synthase (CS), and glycerol kinase (GK) in <i>T. cruzi</i> epimastigotes expressing H2B.V-Myc as a nuclear marker.
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HK, CS, and GK were detected with Alexa Fluor 488-conjugated secondary antibody, H2B.V-Myc was detected with Alexa Fluor 555-conjugated secondary antibody, and DNA was stained with DAPI. Images were acquired using an Olympus BX51 fluorescence microscope controlled by Olympus cell^R software and processed/assembled using ImageJ. Bright-field, individual fluorescence channels, and merged images are shown.
White arrows indicate the linear regions of interest drawn across the parasite body and nuclear region.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pntd.0013299.g005 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Bioinformatics and computational biology · Chemistry · Earth & Environmental Science · Gene expression · Genetics · Life Sciences · Medicine & Health · Physics
- Inferred from text
- Image 65%
Provenance · 1 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34017761 | 11 d ago | JSON v1 |
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| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3102 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Computational Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:chemistry | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
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| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:physics | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |